Molecular based methods like PCR, Real-Time PCR and more recently next generation sequencing (NGS) have revolutionized the field of veterinary diagnostics.
At MBG, we offer detection of pathogenic viruses, bacteria and parasites using molecular methods, which are fast and highly sensitive to detect microbial
pathogens in various specimens.
MBG is an ISO 17025 accredited facility and benefits from an isolated containment level 3 facility for processing highly (level 3) contagious pathogens.
African horse sickness (AHS) virus is a double stranded RNA virus of the family Reoviridae and causes African horse sickness in horses and related species such as mules, donkeys, and zebras. Horses are
most severely affected by the disease. AHS is endemic in the central tropical regions of Africa and has
occasionally extended to Egypt, the Middle East and the southern Arabian Peninsula.
The disease spreads mainly by the bite of insects such as midges (Culicoides) but direct horse to horse
transmission does not occur. The virus can only survive through continuous cycles of transmission
between its hosts-horses and insects. It does not survive in the environment outside of the host. Clinical signs are typically seen five to seven days after infection in the form of fever, redness of the inside surface of the eyelids. The disease progresses to one of the following forms: pulmonary form, cardiac form or mixed form.
There are nine distinct serotypes of AHSV (AHSV-1 to AHSV-9) . Any of the strains can cause disease
with severity ranging from a mild fever to sudden death. The serotypes can be distinguished in serum
neutralisation tests by the specificity of their reactions with neutralising antibodies. Molecular methods for determination of AHSV serotypes have been developed based on a set of nine individual ‘typing’ assays to detect and identify Seg-2 of each AHSV serotype.
Samples should be transported at 4°C and delivered within 24h of collection
Turn Around Time (TAT)
Normal Turnaround time for pathogen Identification is within 5 working days. Urgent Samples will be reported within half of the minimum test period & will be Charged Double.
Samples delivered after 11:00 AM will be processed next working day unless urgent.
The genus Anaplasma belonging to the Anaplasmataceae family (order Rickettsiales) comprises obligate intracellular Gram-negative bacteria is one of the predominant tick borne pathogens of veterinary and public health importance.
Anaplasma exhibit a biological cycle involving infection of both invertebrate and vertebrate host with different cell types targeted mainly infection of the red blood cells. Ticks transmit pathogens that cause disease thorough the process of feeding and the incubation period is 5-14 days. Affecting species are ruminants, dogs, horses, camels and also in human (Human granulocytic anaplasmosis). General systemic signs include anaemia, diarrhea, anorexia, and weight loss.
In the initial phase of infection, hosts are usually seronegative and serological cross-reactions with several Anaplasma species are observed. So, in terms of sensitivity and specificity PCR tests are the main approach for a case definition and epidemiological studies compared to other laboratory test methods.
In MBG, instead of detecting closely related species, we have been designed, a genus-specific primers to amplify a conserved region that target entire Anaplasma genus. One of the reasons is that some Anaplasma species are ecologically divergent and not found in the same hosts or vectors.
Diagnosis for sensitive and specific identification of Anaplasma infections usually performed on total DNA from different kind of sample types including blood, culture but in persistently infected animals with intermittent or low-level bacteremia, other tissues might be useful.
Method
PCR & Gel Electrophoresis
Sample Type
EDTA Blood
Transport Condition
Sample should be transported at 4oC. (Refer to MBG-C0014)
Turn Around Time (TAT)
The Turnaround (TAT) for routine samples is within 3 working days.
Samples delivered before 11:00 AM will begin processing immediately resulting in shorter TAT.
Urgent Samples must be delivered before 11:00 AM and will be charged double.
Aspergillus spp. has been found worldwide in almost all domestic and wild animals, ranging from insects and corals to NHP. Aspergillosis is a common infection in birds, particularly pet parrots, penguins, captive raptors, mallards and other ducks, turkeys, and Japanese quail, in which it causes pulmonary and air sac infection. Aspergillosis has also been described in cats and dogs in which it affects only the nasal passages. In large animals, Aspergillus infection is assumed to be rare but has been reported with increasing frequency. It can lead to various diseases like mycotic abortion and gland infection in cows as well as guttural pouch involvement in horses. In marine mammals, airways are usually initially affected leading to pneumonia, but other organs including the brain may also be infected following fatal dissemination. The most frequently identified pathogens are Aspergillus fumigatus and Aspergillus flavus, ubiquitous organisms capable of living under extensive environmental stress.
Aspergillus fumigatus is an opportunistic pathogen and the primary cause of invasive aspergillosis. Triazole antifungals are used to treat aspergillosis. However, triazole-resistant A. fumigatus infections are increasingly reported worldwide and are associated with increased treatment failure and mortality. This fungus can acquire resistance to azole antifungals due to mutations in the azole target (Cyp51A). Cyp51A mutations typical for environmental azole resistance acquisition (for example, TR34/L98H) have been reported in many cases. These mutations can also be found in isolates recovered from patients.
Pathogens Tested
APA-098 :Aspergillus fumigatus/ terreus/ flavus
APA-189 : Azole-resistance profiling of Cyp51A gene
Method
Aspergillus fumigatus / terreus / flavus by Multi Real-Time PCR (This assay includes the detection and differentiation of Aspergillus fumigatus / terreus / flavus) Azole-resistance profiling of Cyp51A gene(This assay detects selected mutations in the Cyp51A gene related to azole-resistance, by PCR and sequencing).
Normal Turnaround time for pathogen Identification is within 5 working days. Urgent Samples will be reported within half of the minimum test period & will be Charged Double.
Samples delivered after 11:00 AM will be processed next working day unless urgent.
The adenoviruses are a group of non-enveloped, icosahedral DNA viruses. Adenovirus infections are ubiquitous in commercially farmed birds, and probably in all avian species.
The avian adenoviruses can be divided into three groups. Group I, or conventional adenoviruses, share a common group antigen. Group II adenoviruses include the viruses of turkey haemorrhagic enteritis (THE), marble spleen disease (MSD) and group II splenomegaly of chickens. These viruses also share a common antigen. Group III viruses, the egg drop syndrome (EDS) viruses, are widely distributed in waterfowl but can easily infect chickens, resulting in the production of abnormal eggshells. While many infections are subclinical and appear to be of little economic or welfare importance, significant outbreaks of disease associated with adenovirus do occur.
Samples should be transported at 4°C and delivered within 24h of collection.
Turn Around Time (TAT)
Normal Turnaround time for pathogen Identification is within 5 working days. Urgent Samples will be reported within half of the minimum test period & will be Charged Double.
Samples delivered after 11:00 AM will be processed next working day unless urgent.
Molecular based methods like PCR, Real-Time PCR and more recently next generation sequencing (NGS) have revolutionized the field of veterinary diagnostics.
At MBG, we offer detection of pathogenic viruses, bacteria and parasites using molecular methods, which are fast and highly sensitive to detect microbial
pathogens in various specimens.
MBG is an ISO 17025 accredited facility and benefits from an isolated containment level 3 facility for processing highly (level 3) contagious pathogens.
African horse sickness (AHS) virus is a double stranded RNA virus of the family Reoviridae and causes African horse sickness in horses and related species such as mules, donkeys, and zebras. Horses are
most severely affected by the disease. AHS is endemic in the central tropical regions of Africa and has
occasionally extended to Egypt, the Middle East and the southern Arabian Peninsula.
The disease spreads mainly by the bite of insects such as midges (Culicoides) but direct horse to horse
transmission does not occur. The virus can only survive through continuous cycles of transmission
between its hosts-horses and insects. It does not survive in the environment outside of the host. Clinical signs are typically seen five to seven days after infection in the form of fever, redness of the inside surface of the eyelids. The disease progresses to one of the following forms: pulmonary form, cardiac form or mixed form.
There are nine distinct serotypes of AHSV (AHSV-1 to AHSV-9) . Any of the strains can cause disease
with severity ranging from a mild fever to sudden death. The serotypes can be distinguished in serum
neutralisation tests by the specificity of their reactions with neutralising antibodies. Molecular methods for determination of AHSV serotypes have been developed based on a set of nine individual ‘typing’ assays to detect and identify Seg-2 of each AHSV serotype.
Samples should be transported at 4°C and delivered within 24h of collection
Turn Around Time (TAT)
Normal Turnaround time for pathogen Identification is within 5 working days. Urgent Samples will be reported within half of the minimum test period & will be Charged Double.
Samples delivered after 11:00 AM will be processed next working day unless urgent.
The genus Anaplasma belonging to the Anaplasmataceae family (order Rickettsiales) comprises obligate intracellular Gram-negative bacteria is one of the predominant tick borne pathogens of veterinary and public health importance.
Anaplasma exhibit a biological cycle involving infection of both invertebrate and vertebrate host with different cell types targeted mainly infection of the red blood cells. Ticks transmit pathogens that cause disease thorough the process of feeding and the incubation period is 5-14 days. Affecting species are ruminants, dogs, horses, camels and also in human (Human granulocytic anaplasmosis). General systemic signs include anaemia, diarrhea, anorexia, and weight loss.
In the initial phase of infection, hosts are usually seronegative and serological cross-reactions with several Anaplasma species are observed. So, in terms of sensitivity and specificity PCR tests are the main approach for a case definition and epidemiological studies compared to other laboratory test methods.
In MBG, instead of detecting closely related species, we have been designed, a genus-specific primers to amplify a conserved region that target entire Anaplasma genus. One of the reasons is that some Anaplasma species are ecologically divergent and not found in the same hosts or vectors.
Diagnosis for sensitive and specific identification of Anaplasma infections usually performed on total DNA from different kind of sample types including blood, culture but in persistently infected animals with intermittent or low-level bacteremia, other tissues might be useful.
Method
PCR & Gel Electrophoresis
Sample Type
EDTA Blood
Transport Condition
Sample should be transported at 4oC. (Refer to MBG-C0014)
Turn Around Time (TAT)
The Turnaround (TAT) for routine samples is within 3 working days.
Samples delivered before 11:00 AM will begin processing immediately resulting in shorter TAT.
Urgent Samples must be delivered before 11:00 AM and will be charged double.
Aspergillus spp. has been found worldwide in almost all domestic and wild animals, ranging from insects and corals to NHP. Aspergillosis is a common infection in birds, particularly pet parrots, penguins, captive raptors, mallards and other ducks, turkeys, and Japanese quail, in which it causes pulmonary and air sac infection. Aspergillosis has also been described in cats and dogs in which it affects only the nasal passages. In large animals, Aspergillus infection is assumed to be rare but has been reported with increasing frequency. It can lead to various diseases like mycotic abortion and gland infection in cows as well as guttural pouch involvement in horses. In marine mammals, airways are usually initially affected leading to pneumonia, but other organs including the brain may also be infected following fatal dissemination. The most frequently identified pathogens are Aspergillus fumigatus and Aspergillus flavus, ubiquitous organisms capable of living under extensive environmental stress.
Aspergillus fumigatus is an opportunistic pathogen and the primary cause of invasive aspergillosis. Triazole antifungals are used to treat aspergillosis. However, triazole-resistant A. fumigatus infections are increasingly reported worldwide and are associated with increased treatment failure and mortality. This fungus can acquire resistance to azole antifungals due to mutations in the azole target (Cyp51A). Cyp51A mutations typical for environmental azole resistance acquisition (for example, TR34/L98H) have been reported in many cases. These mutations can also be found in isolates recovered from patients.
Pathogens Tested
APA-098 :Aspergillus fumigatus/ terreus/ flavus
APA-189 : Azole-resistance profiling of Cyp51A gene
Method
Aspergillus fumigatus / terreus / flavus by Multi Real-Time PCR (This assay includes the detection and differentiation of Aspergillus fumigatus / terreus / flavus) Azole-resistance profiling of Cyp51A gene(This assay detects selected mutations in the Cyp51A gene related to azole-resistance, by PCR and sequencing).
Normal Turnaround time for pathogen Identification is within 5 working days. Urgent Samples will be reported within half of the minimum test period & will be Charged Double.
Samples delivered after 11:00 AM will be processed next working day unless urgent.
The adenoviruses are a group of non-enveloped, icosahedral DNA viruses. Adenovirus infections are ubiquitous in commercially farmed birds, and probably in all avian species.
The avian adenoviruses can be divided into three groups. Group I, or conventional adenoviruses, share a common group antigen. Group II adenoviruses include the viruses of turkey haemorrhagic enteritis (THE), marble spleen disease (MSD) and group II splenomegaly of chickens. These viruses also share a common antigen. Group III viruses, the egg drop syndrome (EDS) viruses, are widely distributed in waterfowl but can easily infect chickens, resulting in the production of abnormal eggshells. While many infections are subclinical and appear to be of little economic or welfare importance, significant outbreaks of disease associated with adenovirus do occur.
Samples should be transported at 4°C and delivered within 24h of collection.
Turn Around Time (TAT)
Normal Turnaround time for pathogen Identification is within 5 working days. Urgent Samples will be reported within half of the minimum test period & will be Charged Double.
Samples delivered after 11:00 AM will be processed next working day unless urgent.
